Long Re(ad)volution workshop
1.1
  • Setting up your de.NBI Cloud SimpleVM instance
  • Command Line Introduction: Excercises
  • The Tutorial Data Set
  • Basecalling
  • Data Quality Assessment
  • Assembly
  • Assembly polishing
  • Genome annotation
Long Re(ad)volution workshop
  • Welcome to the de.NBI Long Read Training Course!
  • View page source

Welcome to the de.NBI Long Read Training Course!

Welcome to the two-day nanopore training course. This tutorial will guide you through the typical steps of a nanopore assembly of a microbial genome.

  • Setting up your de.NBI Cloud SimpleVM instance
  • Command Line Introduction: Excercises
    • Excercise 1.1: Directory structure
    • Excercise 1.2: Directory structure
    • Excercise 2: Tab completion
    • Excercise 3: Basic File Operations
    • Excercise 4: Links
    • Excercise 5: Display File Content
    • Excercise 6: Wildcards
    • Excercise 7: grep and wc
    • Excercise 8: Streams
    • Excercise 9: Tabular Data
    • Excercise 10: Shell scripts
  • The Tutorial Data Set
  • Basecalling
    • Inspect the fast5 files
    • Basecalling with dorado
    • Inspect the output
    • The results with complete data
    • Merge fastqs
  • Data Quality Assessment
    • FastQC
    • Generating Error Profiles
  • Assembly
    • Assembly with flye
    • Assembly evaluation with QUAST
    • Assembly Graph inspection with bandage
    • Quality control by mapping
  • Assembly polishing
    • Assembly polishing with pilon
    • Assembly polishing with racon and medaka
    • Assembly polishing with nanopolish
  • Genome annotation
    • Annotating the genome with Bakta
    • Comparative genomics with EDGAR
Next

© Copyright 2025, Alexander Sczyrba.

Built with Sphinx using a theme provided by Read the Docs.